Detail publikace

Evaluations of methods for DNA isolation from plant samples

TROJÁNEK, Z., HÚSKA, D., ŠPANOVÁ, A., KOVAŘÍK, A., RITTICH, B.

Originální název

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

The aim of this study was to evaluate CTAB extraction procedure, Qiagen DNA easy kit, direct homogenization, carboxyl-functionalised magnetic non-porous HEMA based microspheres and combination of the above mentioned methods for DNA isolation from different plants. The DNA was evaluated regarding concentration and purity, relative intactness, amplification in PCR. All methods provided DNA that could be used in downstream PCR applications. Here we provide an optimized protocol for isolation of DNA from different plant species by combination of direct homogenization and magnetic microspheres coated by carboxyl groups.

Klíčová slova

magnetic particles, plants, foods, polymerase chain reaction, identification

Autoři

TROJÁNEK, Z., HÚSKA, D., ŠPANOVÁ, A., KOVAŘÍK, A., RITTICH, B.

Vydáno

28. 5. 2013

Nakladatel

Institute of Immunolology and Experimental Therapy PASci

Místo

Polsko

ISBN

978-83-928488-3-7

Kniha

Microbiology and Immunology of Mucosa Probiotics Conference 2013

Číslo edice

Strany od

Strany do

Strany počet

BibTex

@misc{BUT100203,
  author="Bohuslav {Rittich}",
  title="Evaluations of methods for DNA isolation from plant samples",
  booktitle="Microbiology and Immunology of Mucosa Probiotics Conference 2013",
  year="2013",
  edition="1",
  pages="45--45",
  publisher="Institute of Immunolology and Experimental Therapy PASci",
  address="Polsko",
  isbn="978-83-928488-3-7",
  note="abstract"
}