Detail předmětu
Advanced Techniques of Thermal Analysis
FCH-DA_PTTAk. rok: 2022/2023
Fluorescence spectroscopy. Fluorescence microscopy. Flow cytometry. Fluorescence correlation spectroscopy (FLIM, FCS). Technical aspects and interpretation of particular advanced fluorescence techniques. Preparation of model system (associative colloid system), analysis and data interpretation. Techniques: time resolved emission spectrocsopy, anisotrophy, fluorescence correlation spectroscopy, fluorescence bleaching, corss-correlation spectroscopy
Jazyk výuky
angličtina
Garant předmětu
Zajišťuje ústav
Způsob a kritéria hodnocení
Study results are verified pursuant to Article 34, Paragraph 7 of Study and Examination Rules of BUT. Students are given a credit for submitting protocols for each technique they studied. Protocols are processed in the following format: principles of the technique, the aim and exact methods used for measurement, collected data, their evaluation and interpretation, assessment of the impact of the technique on biophysical practice.
Osnovy výuky
Fluorescence spectroscopy. Fluorescence microscopy. Flow cytometry. Fluorescence correlation spectroscopy (FLIM, FCS). Technical aspects and interpretation of particular advanced fluorescence techniques. Preparation of model system (associative colloid system), analysis and data interpretation. Techniques: time resolved emission spectrocsopy, anisotrophy, fluorescence correlation spectroscopy, fluorescence bleaching, corss-correlation spectroscopy
Učební cíle
Fluorescence spectroscopy. Fluorescence microscopy. Flow cytometry. Fluorescence correlation spectroscopy (FLIM, FCS). Technical aspects and interpretation of particular advanced fluorescence techniques. Preparation of model system (associative colloid system), analysis and data interpretation. Techniques: time resolved emission spectrocsopy, anisotrophy, fluorescence correlation spectroscopy, fluorescence bleaching, corss-correlation spectroscopy
Základní literatura
Denes, L. The Nature of Biological Systems as Revealed by Thermal Methods (Hot Topics in Thermal Analysis and Calorimetry). Netherlands: Springer, 2005 (EN)
Privalov, P.L. Microcalorimetry of macromolecules: The Physical Basis of Biological Structures. N. York: Wiley, 2012 (EN)
Privalov, P.L. Microcalorimetry of macromolecules: The Physical Basis of Biological Structures. N. York: Wiley, 2012 (EN)