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Všech fakult

By completion of the course the student will gain the following knowledge and skills:
1. They will know in depth the principles of simple instrumental analytical methods and possibilities of their application.
2. They will be able to select the optimal method for solving of specific analytical problems of analytical practice.
3. They will adopt the basic principles of chemometric treatment of analytical results obtained using simple instrumental analytical methods.

Knowledge from the course Analytical chemistry I, knowledge from physics (electricity, magnetism), knowledge from physical chemistry

The course uses teaching methods in form of Lecture - 2 teaching hours per week, seminars - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Course credit is awarded for 100% attendance at seminars (any absence must be properly apologized). Credit may be obtained for successful completing of three partial written tests during the semester (i.e. getting at least 6 points out of 10 possible). In case of failure in one partial test (or more) course credit could be awarded for the successful completing of the final test (i.e. obtaining at least 60 points out of 100).
Condition for passing the exam is to obtain credit from the exercise of Analytical Chemistry II.
The exam is written test, evaluate the level of knowledge of theory and practice of analytical chemistry and creative approach to solving analytical problems.

1. Introduction to instrumental analysis. Position and importance of intrumental analytical techniques, their historical development, definition of basic concepts.

Topic I: Electroanalytical Methods
2. Potentiometry
3. Voltammetry and polarography (classical, differential pulse, dissolution, adsorption). Potentiometric dissolution analysis, titration with polarizable electrodes. Electrogravimetry, Coulometry, Conductometry.

Topic II: Spectrometry
4. Classification of spectral methods, basic physical principles, properties of electromagnetic radiation. Non-spectral methods: Refractometry, interferometry, polarimetry, nephelometry, turbidimetry. UV-VIS spectrophotometry - principle, properties, device design, application.
5. Luminescent methods: molecular fluorescence, phosphorescence, chemiluminescence, bioluminescence and their analytical use.
6. Mass spectrometry.
7. Fundamentals of optical atomic spectrometry.

Topic III: Separation Methods
Basic theory of chromatographic separation process. Planar Chromatography, Column Chromatography.
9. Liquid chromatography
10. Gas chromatography.
11. Separation in the electric field. Planar techniques (gel electrophoresis), capillary techniques (capillary zone electrophoresis, isotachophoresis, capillary isoelectric focusation).
12. Tandem techniques.

Topic IV: Evaluation of results
13. Basics of analytical chemometry. Procedures for evaluating analytical results and calibration functions.

The aim of the course is to provide students with an overview of selected instrumental analytical methods from the field of electrochemistry, spectrophotometry and separation techniques and to familiarize them with their application fields and their limitations.

Attendance at lectures is recommended, but not checked. Attendance at seminars is compulsory and is checked.
The students' work is controlled by three partial written tests during the semester in computational exercises.
For students in combined form of study are organized consultations on the topics for full-time students.
Consultations are also provided to full-time students on request.

Christian G.D.: Analytical chemistry. Wiley, 2003. (EN)
Khopkar S.M.: Basic concepts of analytical chemistry. New Age International, Delhi 1998. (EN)
Settle, F.A.: Handbook of Instrumental Techniques for Analytical Chemistry. Prentice Hall PTR, 1997. (EN)