Determination of antibiotics in wastewater

audiovizuální tvorba

angličtina

Antibiotics are widely used in human and veterinary medicine for a bacterial infection treatment. These compounds have been shown to block biological processes in wastewater treatment plants. They harm natural ecosystems and they may concentrate in food chain. Furthermore, the bacterial resistence against them has been proven to establish easily. Sulfonamides (sulfathiazole) and tetracyclines (tetracycline, chlortetracycline hydrochloride) were determined in this work. Sulphonamides are the derivates of p aminobenzensulphonamide and the first used antibacterial pharmaceuticals. The analysis of sulphonamides is influenced by their properties, namely insolubility in water, alcohol, ether and dichloromethane. The samples were filtered, extracted by using ethylacetate and evaporated by a rotary vacuum evaporator. The evaporation residues were dissolved in methanol and in a mobile phase. The mixture of acetonitrile and 0.1% formic acid in purified water were used as the mobile phase. The samples were filtered by membrane filters (0.45 m) into the amber glass vials. The limit of detection was 0.3 ng.ml-1. Tetracyclines are produced by Streptomyces. They are fotosensitive and unstable in water solutions. They block aminoacids incorporation into the peptide chains. The samples for tetracyclines determination were sonicated in the McIlvain buffer and concentrated by solid phase extraction (C18). The samples were filtered by the membrane filters (0.45 m) into the amber glass vials. Sulphuric acid and acetonitrile were used as the mobile phase for tetracyclines. The limit of detection was 0.03 ng.ml-1. The HPLC instrument equipped with a diode array detector was used for all compounds determination.

antibiotics, HPLC, wastewater

VÍTEČKOVÁ, H.; VÁVROVÁ, M.; MRAVCOVÁ, L.

15. 6. 2007

I.O.M.P.S.

Ghent

675

675

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