Course detail
Bioanalytical Methods
FCH-MC_BIMAcad. year: 2023/2024
Not applicable.
Language of instruction
Czech
Number of ECTS credits
4
Mode of study
Not applicable.
Guarantor
Entry knowledge
Not applicable.
Rules for evaluation and completion of the course
Examination is oral
Attendance at lectures is not obligatory but it is recommended
Attendance at lectures is not obligatory but it is recommended
Aims
The aim is to acquire knowledge about the methods that are used for analysis of biological materials.
Study aids
Not applicable.
Prerequisites and corequisites
Not applicable.
Basic literature
Záruba, Kamil. Analytická chemie. Praha: VSCHT v Praze, 2016. (CS)
Manz, A. Bioanalytical chemistry. London: Imperial College Press, 2015. (EN)
Manz, A. Bioanalytical chemistry. London: Imperial College Press, 2015. (EN)
Recommended literature
Ramesh, Vasudevan. Biomolecular and Bioanalytical Techniques. Wiley, 2019. (EN)
UGO, Paolo and Coll. Bioanalytical Chemistry. De Gruyter, 2021. (EN)
UGO, Paolo and Coll. Bioanalytical Chemistry. De Gruyter, 2021. (EN)
Elearning
eLearning: currently opened course
Classification of course in study plans
- Programme NKCP_CHPL Master's 2 year of study, winter semester, compulsory-optional
- Programme NPCP_CHMA Master's
specialization BF , 2 year of study, winter semester, compulsory-optional
specialization CHBL , 2 year of study, winter semester, compulsory-optional - Programme NPCP_CHPL Master's 2 year of study, winter semester, compulsory-optional
- Programme NPCP_PCHBT Master's 2 year of study, winter semester, compulsory-optional
Type of course unit
Lecture
26 hod., optionally
Teacher / Lecturer
Syllabus
1) Introduction to bioanalytical methods and their application
2) Overview of bioanalytical techniques, basic principles of biopolymer analysis
3) Enzymology - Enzyme distribution, enzyme kinetics, enzymes as analytical reagents
4) Enzymology - immobilization of enzymes, use in practice
5) Immunochemical Methods - Principles and Overview of Antibody Techniques
6) Immunochemical methods - fluorescence, bioluminescence, radioactive and enzyme immunoassay
7) Nanotechnology, biosensors, construction and applications
8) Chromatographic techniques - gel permeation, affinity and ion exchange chromatography, gas (GC) and liquid chromatography (HPLC, UHPLC), supercritical fluid chromatography (SFC) and their use in biomolecular analysis
9) Spectrophotometric and microscopic methods - optical, fluorescence, infrared, raman and high-frequency spectroscopy, nuclear magnetic resonance, atomic absorption and emission spectrometry
10) Electrophoretic methods - electrophoresis, isoelectric focusing, isotachophoresis, affinity, two-dimensional, zonal and capillary electrophoresis.
11) Molecular techniques, polymerase chain reaction (PCR), RT-PCR detection of specific product sequences in situ, DNA and RNA chips, examples of practical use
12) Biological and microbiological analytical methods, flow cytometry, fluorescence techniques, electron microscopy
13) Modern bioanalytical techniques
2) Overview of bioanalytical techniques, basic principles of biopolymer analysis
3) Enzymology - Enzyme distribution, enzyme kinetics, enzymes as analytical reagents
4) Enzymology - immobilization of enzymes, use in practice
5) Immunochemical Methods - Principles and Overview of Antibody Techniques
6) Immunochemical methods - fluorescence, bioluminescence, radioactive and enzyme immunoassay
7) Nanotechnology, biosensors, construction and applications
8) Chromatographic techniques - gel permeation, affinity and ion exchange chromatography, gas (GC) and liquid chromatography (HPLC, UHPLC), supercritical fluid chromatography (SFC) and their use in biomolecular analysis
9) Spectrophotometric and microscopic methods - optical, fluorescence, infrared, raman and high-frequency spectroscopy, nuclear magnetic resonance, atomic absorption and emission spectrometry
10) Electrophoretic methods - electrophoresis, isoelectric focusing, isotachophoresis, affinity, two-dimensional, zonal and capillary electrophoresis.
11) Molecular techniques, polymerase chain reaction (PCR), RT-PCR detection of specific product sequences in situ, DNA and RNA chips, examples of practical use
12) Biological and microbiological analytical methods, flow cytometry, fluorescence techniques, electron microscopy
13) Modern bioanalytical techniques
Guided consultation in combined form of studies
26 hod., optionally
Teacher / Lecturer
Elearning
eLearning: currently opened course